run("Close All"); // open intensity image open("https://github.com/NEUBIAS/training-resources/raw/master/image_data/xy_8bit__two_cells.tif"); rename("intensities"); // create label mask image setThreshold(49, 255); setOption("BlackBackground", true); run("Analyze Particles...", " show=[Count Masks]"); rename("labels"); setMinAndMax(0, 2); // open with SegmentationAnnotator run("Open Intensity and Label Mask Image...", "intensityimage=intensities labelimage=labels");